Journal: Frontiers in cardiovascular medicine

Article Title: Deficiency of a novel lncRNA-HRAT protects against myocardial ischemia reperfusion injury by targeting miR-370-3p/RNF41 pathway.

doi: 10.3389/fcvm.2022.951463

Figure Lengend Snippet: FIGURE 3 Characterization of lncRNA-HRAT. (A) Assessment the protein-coding potential of lncRNA-HRAT by Coding Potential Calculator Online. (B) Schematic annotation of the lncRNA-HRAT genomic locus. (C) The analysis of Adora1 expression in Ctrl and H/R cells was conducted through qRT-PCR (n = 3). (D) The analysis of Adora1 expression in H9c2 cells infected with lncRNA-HRAT or control lentivirus was conducted through qRT-PCR (n = 3). (E) The localization of lncRNA-HRAT in H9c2 cells was tested via RNA FISH (n = 3). Nuclei was stained with 40,6-diamidino-2-phenylindole (DAPI; blue), 18S and lncRNA-HRAT appeared red. Scale bar, 10µm. (F) The analysis of lncRNA-HRAT expression in the nucleus and cytoplasm of H9c2 cells was employed through qRT-PCR (n = 3). The cytoplasmic control was GAPDH and β-actin, while the nuclear control was U6. All the data are described as the mean ± SD. *P < 0.05.

Article Snippet: After blocking with 5% milk at RT, the membrane was then incubated with antibodies against βactin (1:5000, Proteintech, 20536-1-AP), GAPDH (1:1000; Proteintech, 10494-1-AP), Caspase-3 (1:1000, CST, 9662S), cleaved Caspase-3 (1:1000, CST, 9664S), RNF41 (1:1000, Proteintech, 17233-1-AP) overnight at 4◦C. β-actin and GAPDH antibody was served as control.

Techniques: Expressing, Quantitative RT-PCR, Infection, Control, Staining

Journal: Nutrients

Article Title: Lentil Waste Extracts for Inflammatory Bowel Disease (IBD) Symptoms Control: Anti-Inflammatory and Spasmolytic Effects.

doi: 10.3390/nu16193327

Figure Lengend Snippet: Figure 2. Effects of different lentil hull extracts on TLR4 expression levels in Caco-2 cells treated with LPS (1 µg/mL) for 24 h. Immunoblotting detection in the Caco-2 cells of control, LPS, BEVa and BEVa plus LPS (A), BEVb and BEVb plus LPS (B), mBEVa and mBEVa plus LPS (C), and mBEVb and mBEVb plus LPS (D). Densitometric analysis of TLR4 expression, after normalization against β-actin, is reported. Data are presented as means ± SD of five independent experiments. (*** p < 0.001 vs. control; ### p < 0.001, ## p < 0.01, and # p < 0.05 vs. LPS).

Article Snippet: Then, following a standard avidin-biotin complex procedure, membranes were incubated in the dark with primary mouse monoclonal antibody (moAb) anti-βactin (sc-47778), rabbit poAb anti-TLR4 (sc-10741) (all from Santa Cruz Biotechnology, Inc., Milan, Italy), at a 1:500 dilution.

Techniques: Expressing, Western Blot, Control

Journal: Nutrients

Article Title: Lentil Waste Extracts for Inflammatory Bowel Disease (IBD) Symptoms Control: Anti-Inflammatory and Spasmolytic Effects.

doi: 10.3390/nu16193327

Figure Lengend Snippet: Figure 3. Analysis of pro-inflammatory responses. Real-time PCR analysis of iNOS mRNA expression levels in Caco2-untreated cells (control), treated with LPS alone (LPS) or treated with LPS after pre- treatment with different lentil hull extracts: (A) BEVa and mBEVa and (B) BEVb and mBEVb. Values represent the mRNA fold changes relative to β-actin used as resident control and expressed as means ± SD of five independent experiments. (** p < 0.05 vs. control; ## p < 0.01 and # p < 0.05 vs. LPS).

Article Snippet: Then, following a standard avidin-biotin complex procedure, membranes were incubated in the dark with primary mouse monoclonal antibody (moAb) anti-βactin (sc-47778), rabbit poAb anti-TLR4 (sc-10741) (all from Santa Cruz Biotechnology, Inc., Milan, Italy), at a 1:500 dilution.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Control

Journal: Nutrients

Article Title: Lentil Waste Extracts for Inflammatory Bowel Disease (IBD) Symptoms Control: Anti-Inflammatory and Spasmolytic Effects.

doi: 10.3390/nu16193327

Figure Lengend Snippet: Figure 4. Analysis of pro-inflammatory IL-1 cytokine expression. Real-time PCR analysis of IL-1 mRNA expression levels in Caco-2 untreated cells (Control), treated with LPS alone (LPS) or treated with LPS after pre-treatment of different lentil peel extracts: (A) BEVa and mBEVa and (B) BEVb and mBEVb. Values represent the mRNA fold changes relative to β-actin used as resident control and expressed as means ± SD of five independent experiments. (*** p < 0.001 and ** p < 0.01 vs. control; ### p < 0.001, ## p < 0.01, and # p < 0.05 vs. LPS).

Article Snippet: Then, following a standard avidin-biotin complex procedure, membranes were incubated in the dark with primary mouse monoclonal antibody (moAb) anti-βactin (sc-47778), rabbit poAb anti-TLR4 (sc-10741) (all from Santa Cruz Biotechnology, Inc., Milan, Italy), at a 1:500 dilution.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control

Journal: Nutrients

Article Title: Lentil Waste Extracts for Inflammatory Bowel Disease (IBD) Symptoms Control: Anti-Inflammatory and Spasmolytic Effects.

doi: 10.3390/nu16193327

Figure Lengend Snippet: Figure 5. Analysis of anti-inflammatory cytokine expression. Real-time PCR analysis of IL-10 mRNA expression levels in Caco-2 untreated cells (Control), treated with LPS alone (LPS) or treated with LPS after pre-treatment of different lentil hull extracts: (A) BEVa and mBEVa and (B) BEVb and mBEVb. Values represent the mRNA fold changes relative to β-actin used as resident control and expressed as means ± SD of five independent experiments. (*** p < 0.001 and ** p < 0.01 vs. control; ### p < 0.001, ## p < 0.01, and # p < 0.05 vs. LPS).

Article Snippet: Then, following a standard avidin-biotin complex procedure, membranes were incubated in the dark with primary mouse monoclonal antibody (moAb) anti-βactin (sc-47778), rabbit poAb anti-TLR4 (sc-10741) (all from Santa Cruz Biotechnology, Inc., Milan, Italy), at a 1:500 dilution.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control